Temporary

Part:BBa_J331028

Designed by: Gilles Yvan Buck   Group: Buck Lab   (2016-12-13)


Enzymatic Pathway for Desulfurization

Sulfur third most abundant element in crude oil, when hydrocarbons that have sulfur are burnt they release S02 and S03 gases, sulfur reduces the quality of oil while at the same time polluting the earth. We are going to remove the sulfur in petroleum while keeping the hydrocarbon backbone intact because hurting it will have adverse effects on the petroleums quality. We are going to use a 4s pathway found in Rhodococcus spp, we will remove sulfur from the substrate dibenzothiophene (DBT) and convert it to 2-hydroxybiphenyl (2-HBP), a substrate that does not contain sulfur, we are going to do this without breaking the hydrocarbon backbone

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1428
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4993
    Illegal BglII site found at 5928
    Illegal BglII site found at 6055
    Illegal BamHI site found at 652
    Illegal BamHI site found at 2232
    Illegal BamHI site found at 5845
    Illegal XhoI site found at 644
    Illegal XhoI site found at 3384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 359
    Illegal NgoMIV site found at 2673
    Illegal NgoMIV site found at 2888
    Illegal NgoMIV site found at 3192
    Illegal NgoMIV site found at 3312
    Illegal AgeI site found at 2847
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2382
    Illegal SapI.rc site found at 3696
    Illegal SapI.rc site found at 4509


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